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Array-Based Analysis
RNA Labeling
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RNA Labeling

서브텍스트

Aminoallyl-UTP

Aminoallyl-UTP [5-(3-aminoallyl-)UTP; AA-UTP] offers a cost-effective alternative to direct incorporation of a labeled NTP when preparing nonradioactive RNA probes by in vitro transcription for microarray analysis, in situ hybridization, or blotting applications. AA-UTP is readily incorporated into RNA transcripts by T7 RNA Polymerase, producing aminoallyl-RNA. The aminoallyl-RNA can then be conjugated to an amine-reactive biotin, for example Biotin-X-X-NHS, or fluorescent dye to produce the labeled RNA.
Aminoallyl-UTP is supplied in a convenient 50-mM solution.


Biotin-16-UTP

Biotin-16-UTP is a biologically active analog of uridine-5´-triphosphate that is readily incorporated into RNA during an in vitrotranscription reaction by RNA polymerases such as T7, T3, or SP6 RNA Polymerases.

Formulation: Supplied as Li+ salt at 50 mM solution in water.

Molecular Formula: C32H52N7O19P3S (free acid)

Molecular Weight: 963.5 g/mol (free acid)

Extinction Coefficient: 7,100 M-1 cm-1 at 292 nm

Quality Control: Purity >97% by HPLC; Purity >99% by 31P NMR.


i_biotin_16utp.gif




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