RNA Labeling
서브텍스트
Aminoallyl-UTP
Aminoallyl-UTP [5-(3-aminoallyl-)UTP; AA-UTP] offers a cost-effective alternative to direct incorporation of a labeled NTP when preparing nonradioactive RNA probes by in vitro transcription for microarray analysis, in situ hybridization, or blotting applications. AA-UTP is readily incorporated into RNA transcripts by T7 RNA Polymerase, producing aminoallyl-RNA. The aminoallyl-RNA can then be conjugated to an amine-reactive biotin, for example Biotin-X-X-NHS, or fluorescent dye to produce the labeled RNA.
Aminoallyl-UTP is supplied in a convenient 50-mM solution.
Aminoallyl-UTP is supplied in a convenient 50-mM solution.
Biotin-16-UTP
Biotin-16-UTP is a biologically active analog of uridine-5´-triphosphate that is readily incorporated into RNA during an in vitrotranscription reaction by RNA polymerases such as T7, T3, or SP6 RNA Polymerases.
Formulation: Supplied as Li+ salt at 50 mM solution in water.
Molecular Formula: C32H52N7O19P3S (free acid)
Molecular Weight: 963.5 g/mol (free acid)
Extinction Coefficient: 7,100 M-1 cm-1 at 292 nm
Quality Control: Purity >97% by HPLC; Purity >99% by 31P NMR.
