마이페이지 |
Reagents
Instruments
Reagents
NEB
PK Brand
Lab Service
  
Array-Based Analysis
HOME > Reagents > Epicentre
RNA Labeling for Expression Microarrarys
정가 Inquire
로그인을 하시면 금액을 확인 하실 수 있습니다.
모델선택 :
모델수량 :

TargetAmpTM

RNA Amplification for Microarray

TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0

TargetAmp™: Selection Guide | Process | Performance | FAQ | Poster (3 MB PDF)

Applications
  • Amplification and aminoallyl-labeling of mRNA in total RNA of 1-50 cells (10-500 pg RNA) for use in microarray analysis. Ideal for small samples such as laser-capture microdissected cells, biopsy samples, cultured cells, or other minute cell or tissue samples.
 
스크린샷 2013-06-13 오후 2.49.32.png
  Figure 1. The TargetAmp™ Kit 1.0 amplifies RNA from a single cell. A: Laser capture of a single cell from a section of rat dorsal root ganglion (DRG). B: Agarose gel of the ~1-3 µg AA-aRNA obtained using the TargetAmp Kit 1.0 to amplify mRNA in the ~10 pg total RNA isolated from two different laser-captured DRG cells. C: Scatter plot of microarray results obtained when Cy™ dye-labeled aRNA target prepared using the TargetAmp Kit 1.0 was hybridized to a glass slide spotted with rat brain cDNA.

The TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0* generates at least 5-million-fold amplification of the poly(A) RNA contained in a total cellular RNA sample. This amplification is sufficient to generate microgram amounts of aminoallyl-labeled antisense RNA (AA-aRNA; also called AA-cRNA) from the total RNA contained in a single cell. The kit uses an Eberwine-type linear RNA amplification method,1 but incorporates a number of improvements that significantly increase the yields of AA-aRNA obtained, while reducing the level of nonspecific amplification products. The kit is recommended for amplifying mRNA in total RNA from 1 to ~50 cells.
The kit provides all reagents (except reverse transcriptases and RNA purification columns) for generating AA-aRNA. The entire procedure takes only ~4 hours of hands-on time and can be completed in 2 days. Multiple samples can be amplified simultaneously. Once produced, the aminoallyl-aRNA can be readily labeled using biotin-NHS or fluorescent dye-NHS compounds. Biotin-NHS is available from Epicentre.

Benefits
  • The kit generates microgram amounts of AA-aRNA from as little as 10 pg of total cellular RNA (Table 1 and Fig. 1), representing greater than 5,000,000-fold amplification.
  • The entire two-round RNA amplification process can be completed in 2 days and requires only about 4 hours of hands-on time.
  • Multiple RNA samples can be easily amplified simultaneously.
  • Highly reproducible amplification and microarray results.
References
  1. Van Gelder, R.N. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 1663.

 스크린샷 2013-06-13 오후 2.50.29.png Table 1. The TargetAmp™ Kit 1.0 produces microgram amounts of AA-aRNA from as little as 10 pg (one cell) of total RNA.Results represent the average of multiple experiments.

 a. Approximately 5% of rat brain total RNA is poly(A) RNA. 
b. Approximately 2% of HeLa total RNA is poly(A) RNA. 
c. Approximately 1.5%-2% of rat kidney total RNA is poly(A) RNA.
*TargetAmp™ aRNA Amplification Products are covered by intellectual property licensed to Epicentre Technologies Corporation from Johnson & Johnson Pharmaceutical Research & Development, L.L.C., and by intellectual property sublicensed to Epicentre Technologies Corporation from Incyte Corporation.


TargetAmp™ 2-Round aRNA Amplification Kit 2.0

TargetAmp™: Selection Guide | Process | Performance | FAQ | Poster (3 MB PDF)
스크린샷 2013-06-13 오후 2.51.14.png
Applications
  • Production of microgram amounts of unlabeled aRNA from mRNA in total RNA of 1-50 cells (10-500 pg RNA) for microarray-based analysis. Ideal for small samples such as laser-capture microdissected cells, biopsy samples, cultured cells, or other minute cell or tissue samples.
  • Generation of amplified aRNA from small samples for use in RT-PCR studies, or for archiving valuable samples.
The TargetAmp™ 2-Round aRNA Amplification Kit 2.0* amplifies the poly(A) RNA in total RNA samples by at least 5-million-fold. This amplification is sufficient to obtain microgram amounts of unlabeled antisense RNA (aRNA; also called cRNA) from a single cell. The kit uses an Eberwine-type linear amplification method,1but incorporates a number of improvements that significantly increase the yields of aRNA obtained, while reducing the level of nonspecific amplification products.

The kit provides all reagents (except reverse transcriptases and RNA purification columns) for generating unlabeled aRNA. Since each NTP is provided in the kit as a separate solution, researchers can also use published methods or develop new methods to make labeled aRNA by direct incorporation of labeled nucleotides during the final in vitro transcription step.

스크린샷 2013-06-13 오후 2.51.50.png
  Table 1. The TargetAmp™ Kit 2.0 produces high yields of unlabeled aRNA from as little as 10 pg of input total RNA. Results represent the average of multiple experiments.
a. Approximately 5% of rat brain total RNA is poly(A) RNA.
b. Approximately 2% of HeLa total RNA is poly(A) RNA.
c. Approximately 1.5%-2% of rat kidney total RNA is poly(A) RNA.

Benefits
  • The kit generates microgram amounts of unlabeled aRNA from as little as 10 pg of total cellular RNA (Table 1), representing greater than 5,000,000-fold amplification.
  • The entire two-round RNA amplification process can be completed in 2 days and requires only about 4 hours of hands-on time.
  • Multiple RNA samples can be easily amplified simultaneously.
References
  1. Van Gelder, R.N. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 1663.
*TargetAmp™ aRNA Amplification Products are covered by intellectual property licensed to Epicentre Technologies Corporation from Johnson & Johnson Pharmaceutical Research & Development, L.L.C., and by intellectual property sublicensed to Epicentre Technologies Corporation from Incyte Corporation.


TargetAmp™ 2-Round Biotin-aRNA Amplification Kit 3.0

TargetAmp™: Selection Guide | Process | Performance | FAQ | Poster (3 MB PDF)
스크린샷 2013-06-13 오후 2.52.48.png

Applications
  • Amplification and biotin-labeling of mRNA in total RNA of 5-50 cells (50-500 pg RNA) for use in microarray analysis.

The TargetAmp™ 2-Round Biotin-aRNA Amplification Kit 3.0* is the only kit that can produce microgram amounts of biotin-antisense RNA (biotin-aRNA; also called cRNA) from as little as five cells (about 50 pg of total RNA) for microarray studies. The kit uses an Eberwine-type linear RNA amplification method,1 but incorporates a number of improvements that significantly increase the yields of biotin-aRNA obtained, while reducing the level of nonspecific RNA amplification products.
The TargetAmp™ 2-Round Biotin-aRNA Amplification Kit 3.0 provides all reagents (except reverse transcriptases and RNA purification columns) to amplify input RNA from 5 to ~50 cells. The entire procedure takes only ~4 hours of hands-on time and can be completed in 2 days. Multiple samples can be amplified simultaneously.

스크린샷 2013-06-13 오후 2.53.11.png
  Table 1. The TargetAmp™ Kit 3.0 reaction produces high yields of biotin-aRNA from as little as 50 pg of total cellular RNA.Results represent the average of multiple experiments.

a. Approximately 2% of HeLa total RNA is poly(A) RNA.

Benefits
  • The kit generates microgram amounts of biotin-aRNA from as little as five cells (about 50 pg of input total cellular RNA; Table 1).
  • Multiple RNA samples can be easily amplified and labeled simultaneously.
  • Linear RNA amplification process ensures preservation of the transcript profile of the sample.
  • Highly reproducible amplification, labeling, and microarray results (Table 2).
 스크린샷 2013-06-13 오후 2.53.50.png  Table 2. Biotin-aRNA produced by the TargetAmp™ Kit 3.0 allows high-sensitivity transcript detection. Biotin-aRNA was prepared from the specified amount of mouse liver and skeletal muscle total RNAs using the TargetAmp 2-Round Biotin-aRNA Amplification Kit 3.0 and then hybridized to the Illumina®MouseRef-8 BeadChip array in duplicates. Data were imported into BeadStudio (Illumina) for analysis. Genes called "detected" are those with detection p-value <0.05.
References
  1. Van Gelder, R.N. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 1663.
*TargetAmp™ aRNA Amplification Products are covered by intellectual property licensed to Epicentre Technologies Corporation from Johnson & Johnson Pharmaceutical Research & Development, L.L.C., and by intellectual property sublicensed to Epicentre Technologies Corporation from Incyte Corporation.
For research use only. Some applications in which this product may be used may be covered by patents or patent applications applicable in certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a license, depending upon the particular application and country in which the product is used.


TargetAmp™ 1-Round Aminoallyl-aRNA Amplification Kit 101

TargetAmp™: Selection Guide | Process | Performance | FAQ | Poster (3 MB PDF)

Applications
  • Amplification and aminoallyl-labeling of mRNA in total RNA of >2,500 cells (≥25 ng RNA) for use in microarray analysis.
The TargetAmp™ 1-Round Aminoallyl-aRNA Amplification Kit 101 achieves at least 5,000-fold amplification of the poly(A) RNA present in as little as 25 ng of total cellular RNA. The kit uses an improved Eberwine-type linear amplification method1 to generate aminoallyl-labeled antisense RNA (AA-aRNA; also called AA-cRNA) corresponding to mRNA in the sample. This amount of amplification is sufficient to obtain AA-aRNA for use as labeled target for microarray analysis from about 2,500 or more cells (25 ng total RNA).

The kit provides all reagents (except reverse transcriptase and RNA purification columns) for generation of AA-aRNA. The entire procedure takes about 2 hours of hands-on time and can be completed in 1 day, even with multiple samples.
Once produced, the aminoallyl-aRNA can be readily labeled using biotin-NHS or fluorescent dye-NHS compounds. Biotin-NHS is available from Epicentre.

스크린샷 2013-06-13 오후 2.54.50.png
  Table 1. The TargetAmp™ Kit 101 produces high yields of AA-aRNA from as little as 25 ng of input RNA. Results represent the average of multiple experiments.

a. Approximately 2% of HeLa total RNA is poly(A) RNA.

Benefits
  • The kit generates microagram amounts of AA-aRNA from as little as 25 ng of total cellular RNA (Table 1), representing greater than 5,000-fold amplification.
  • The simple, single-tube protocol can be completed in 1 day and requires only about 2 hours of hands-on time.
  • Multiple RNA samples can be easily amplified simultaneously.
  • Microarray results using AA-aRNA produced with the TargetAmp Kit 101 are highly reproducible.
  • The TargetAmp Kit 101 has been validated by the microarray group of a leading pharmaceutical company for aRNA yield, amplification efficiency, AA-aRNA size and quality, and reproducibility of transcript detection.
References
  1. Van Gelder, R.N. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 1663.
*TargetAmp™ aRNA Amplification Products are covered by intellectual property licensed to Epicentre Technologies Corporation from Johnson & Johnson Pharmaceutical Research & Development, L.L.C., and by intellectual property sublicensed to Epicentre Technologies Corporation from Incyte Corporation.


TargetAmp™ 1-Round aRNA Amplification Kit 103

TargetAmp™: Selection Guide | Process | Performance | FAQ | Poster (3 MB PDF)

Applications
  • Production of microgram amounts of unlabeled aRNA from mRNA in total RNA of >2,500 cells (≥25 ng RNA).
  • Generation of unlabeled aRNA for use in RT-PCR, or for other applications.
The TargetAmp™ 1-Round aRNA Amplification Kit 103* produces microgram amounts of unlabeled aRNA (also called cRNA) using as little as 25 ng of total cellular RNA in an improved Eberwine-type linear amplification method.1 This unlabeled aRNA is amplified at least 5,000-fold compared to the amount of poly(A) RNA in the sample. The resulting unlabeled aRNA product can be directly labeled by a variety of methods, or used as a template for making labeled cDNA for use as a target nucleic acid for microarray analysis. Also, since each NTP is provided in the kit as a separate solution, researchers can use published methods or develop new methods to make labeled aRNA by incorporation of labeled nucleotides during the in vitrotranscription step. The entire TargetAmp Kit 103 protocol takes less than 2 hours of hands-on time and can be completed in less than 1 day, even when amplifying multiple RNA samples simultaneously.

Benefits
  • The kit generates microgram amounts of aRNA from as little as 25 ng of input total RNA (Table 1), representing greater than 5,000-fold amplification.
  • Simple, single-tube protocol can be completed in 1 day and requires only about 2 hours of hands-on time.
  • Provides flexible alternatives to aminoallyl-UTP labeling of aRNA, including incorporation of biotin-labeled or dye-labeled nucleotides, synthesis of labeled cDNA using aRNA as template for reverse transcription, or direct labeling with biotin for use with microarrays or other applications.
  • Multiple RNA samples can be easily amplified simultaneously.
  • The TargetAmp Kit 103 has been optimized for aRNA yield, amplification efficiency, and aRNA size and quality.

스크린샷 2013-06-13 오후 2.56.10.png
  Table 1. The TargetAmp™ Kit 103 produces high yields of unlabeled aRNA from as little as 25 ng of total RNA.Results represent the average of multiple experiments.

a. Approximately 2% of HeLa total RNA is poly(A) RNA.

References
  1. Van Gelder, R.N. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 1663.


*TargetAmp™ aRNA Amplification Products are covered by intellectual property licensed to Epicentre Technologies Corporation from Johnson & Johnson Pharmaceutical Research & Development, L.L.C., and by intellectual property sublicensed to Epicentre Technologies Corporation from Incyte Corporation.


TargetAmp™ 1-Round Biotin-aRNA Amplification Kit 105

TargetAmp™: Selection Guide | Process | Performance | FAQ | Poster (3 MB PDF)

Applications
  • Amplification and biotin-labeling of mRNA in total RNA of >2,500 cells (≥25 ng RNA) for use in microarray analysis.
The TargetAmp™ 1-Round Biotin-aRNA Amplification Kit 105* produces microgram amounts of biotin-antisense RNA (biotin-aRNA, also called biotin-cRNA) from as little as 25 ng of total cellular RNA. The kit provides all reagents for the preparation of biotin-aRNA (except reverse transcriptase and RNA purification columns) and uses an improved Eberwine-type linear amplification method1 to achieve at least 5,000-fold amplification of the poly(A) RNA present in a total cellular RNA sample. The entire procedure can be completed in 1 day.

 스크린샷 2013-06-13 오후 2.57.40.png  Table 1. A TargetAmp™ Kit 105 reaction produces high yields of biotin-aRNA from as little as 25 ng of input total RNA.Results represent the average of multiple experiments.

a. Approximately 2% of HeLa total RNA is poly(A) RNA. 

Benefits
  • Simple, single-tube, 6-hour procedure. Prepare biotin-aRNA and begin hybridization the same day.
  • Biotin-aRNA produced by the kit detected more transcripts (genes) than competitive kits and showed high correlation with MAQC TaqMan® data (Fig. 1).
  • Multiple RNA samples can be easily amplified and labeled simultaneously.
  • The kit generates microgram amounts of biotin-aRNA from as little as 25 ng of input total cellular RNA (Table 1).
  • Linear RNA amplification process preserves the transcript profile of the sample.
  • Highly reproducible amplification, labeling, and microarray results.

 c_targetamp_correlationratios.jpg  Figure 1. Correlation of expression ratios obtained from qPCR and from microarrays hybridized to aRNA prepared with the TargetAmp™ 105 kit. X axis, log2 expression ratios determined from Human Genome U133 Plus 2.0 Arrays (Affymetrix) hybridized to targets prepared by the TargetAmp 105 kit; Y axis, log2 expression ratios determined by the MAQC, using TaqMan® assays. The high correlation obtained (r = 0.936) indicates the excellent fidelity of the amplification process.

References
  1. Van Gelder, R.N. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 1663.
*TargetAmp™ aRNA Amplification Products are covered by intellectual property licensed to Epicentre Technologies Corporation from Johnson & Johnson Pharmaceutical Research & Development, L.L.C., and by intellectual property sublicensed to Epicentre Technologies Corporation from Incyte Corporation.
For research use only. Some applications in which this product may be used may be covered by patents or patent applications applicable in certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a license, depending upon the particular application and country in which the product is used.


TargetAmp™-Nano Labeling Kit for Illumina® Expression BeadChip®

TargetAmp™: Selection Guide | Process | Performance | FAQ | Poster (3 MB PDF)

Note: This kit was formerly called the TargetAmp™ Nano-g™ Biotin-aRNA Labeling Kit for the Illumina® System.
The TargetAmp™-Nano Labeling Kit for Illumina® Expression BeadChip® produces micrograms of biotin-aRNA (also called cRNA) from as little as 25 ng of total RNA. The easy 6-hour reaction enables researchers to prepare biotin-aRNA and begin BeadChip hybridizations in the same day. Biotin-aRNA produced by the TargetAmp-Nano Kit provides sensitive gene detection and yields consistent differential gene expression results.

Applications
  • Generation of biotin-aRNA target for use with Illumina® Expression BeadChip® arrays from as little as 25 ng total RNA.

Benefits
  • Sensitive gene detection.
  • Easy, 6-hour procedure yields biotin-aRNA target and enables BeadChip hybridization in the same day.
  • Linear RNA amplification process preserves the relative abundance of the original sample.
  • High yields of biotin-aRNA from as little as 25 ng of total RNA makes the kit ideal for preparing replicates and archiving.

 i_targetampnano_linearrna.gif  Figure 1. Schematic overview of the TargetAmp™-Nano Kit procedure.The easy 6-hour reaction enables researchers to prepare biotin-aRNA target and begin BeadChip® hybridization in the same day.

 스크린샷 2013-06-13 오후 2.59.08.png  Table 1. The TargetAmp™-Nano Labeling Kit for Illumina® Expression BeadChip® arrays produces microgram amounts of biotin-aRNA from as little as 25 ng of input total RNA.Results summarize experiments performed using the HeLa Total RNA control provided in the kit.

 c_targetampnano_genes_detected.gif  Figure 2. Genes detected using biotin-aRNA produced by the TargetAmp™-Nano Labeling Kit for Illumina® Expression BeadChip®. Biotin-aRNA was produced from the indicated amount and source of total RNA and hybridized to either the Illumina® MouseRef-8 Expression BeadChip® (mouse liver and mouse muscle RNA) or the HumanHT-12 v4 Expression BeadChip® (human ovary and human testicle).


TargetAmp™-Pico Labeling Kit for Illumina® Expression BeadChip®

TargetAmp™: Selection Guide | Process | Performance | FAQ | Poster (3 MB PDF)

Application

Prepare biotin-antisense RNA (aRNA) for use with Illumina® Expression BeadChip® arrays from minute amounts of RNA. Ideal for samples such laser-capture microdissected (LCM) tissues, flow-sorted cells, or biopsy tissue.
The TargetAmp™-Pico Labeling Kit for Illumina® Expression BeadChip®* is the only kit that can produce micrograms amounts of biotin-aRNA (also called cRNA) from as little as 50 pg of total RNA for use on Illumina Expression BeadChip arrays. The kit uses a significantly improved Eberwine1 linear RNA amplification method, but incorporates a number of improvements that significantly increase the yields of biotin-aRNA obtained, while reducing the level of nonspecific RNA amplification products.

Benefits
  • Linear RNA amplification process ensures preservation of the transcript profile of the sample.
  • High correlation with results obtained using the TargetAmp™-Nano Labeling Kit for Illumina Expression BeadChip.
  • High yields of biotin-aRNA from as little as 50 pg of total RNA (Table 1).

 스크린샷 2013-06-13 오후 2.59.53.png  Table 1. The TargetAmp™-Pico Labeling Kit for Illumina® Expression BeadChip® arrays produces microgram amounts of biotin-aRNA from as little as 50 pg of input total RNA. Results summarize experiments performed using the HeLa Total RNA control provided in the kit.

 c_targetamppico_genesdetected.gif  Figure 1. Genes detected using biotin-aRNA produced by the TargetAmp™-Pico Labeling Kit for Illumina® Expression BeadChip®. Biotin-aRNA was produced from the indicated amount and source of total RNA and hybridized to the Illumina MouseRef-8 Expression BeadChip.

 c_targetamppico_exp_ratio.gif  Figure 2. Correlation of expression ratios (click to enlarge). Mouse liver/skeletal muscle expression ratios were obtained with biotin-aRNA produced from 100 ng and 100 pg of total RNA using the TargetAmp-Nano™ and TargetAmp-Pico™ kits, respectively.

References

Van Gelder, R.N. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 1663.

*TargetAmp™ aRNA Amplification Products are covered by intellectual property licensed to EPICENTRE Technologies Corporation from Johnson & Johnson Pharmaceutical Research & Development, L.L.C., and by intellectual property sublicensed to EPICENTRE Technologies Corporation from Incyte Corporation.

For research use only. Some applications in which this product may be used may be covered by patents or patent applications applicable in certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a license, depending upon the particular application and country in which the product is used.


RiboMultiplier™ Sense-RNA Amplification Kit 
dsDNA Conversion Kit for RiboMultiplier™ sRNA

Applications
Production of sense RNA (sRNA) from 10-500 ng of total RNA. Use the amplified sRNA for:
  • Archiving valuable RNA samples for future use.
  • Microarray studies requiring either sRNA target or labeled antisense cDNA target for improved 5´/3´ coverage.
  • Real-time PCR studies of gene expression.
Or, convert the amplified sRNA to dsDNA using the dsDNA Conversion Kit for RiboMultiplier™ for:
  • Target for Roche-NimbleGen expression arrays.
  • Preparation of templates for high-throughput (next-gen) sequencing.
  • cDNA library construction.
The RiboMultiplier™ Sense-RNA Amplification Kit* generates amplified sense-strand RNA (sRNA) from the mRNA contained in a total RNA sample (Fig. 1). First-strand cDNA is synthesized from total RNA, and the RNA is hydrolyzed. Next, an oligo-nucleotide containing a terminal-tagging sequence (TTS), a 3´-blocked terminus, and a random sequence is used to generate cDNA molecules with a known sequence tag at their 3´ ends. A double-stranded T7 promoter sequence is then added to the 3´ ends of the cDNA molecules by DNA synthesis, followed by in vitro transcription, which results in greater than 10,000-fold amplification of the original message. The entire sRNA procedure is performed in a single reaction tube with a hands-on time of less than 1 hour.

Benefits
  • sRNA shows excellent correlation with unamplified samples, indicating a faithful representation of the original gene expression profile (Fig. 2).
  • The unique terminal-tagging procedure yields sRNA with an excellent 3´/5´ ratio and less 3´ bias compared to other oligo(dT)-primed RNA amplification processes (Table 1).
  • Microgram amounts of sRNA are generated from as little as 10 ng of input total RNA (Table 2), with essentially no background amplification.
  • The entire RiboMultiplier sRNA kit procedure can be completed in 1 day.

 
i_ribom_srna_fig1.gif
  Figure 1. Schematic overview of the RiboMultiplier Sense-RNA Amplification procedure.

 A.  B.
 
c_ribom_srna_fig2a.gif
  
c_ribom_srna_fig2b.gif


Figure 2. Correlation of differential gene expression data from sRNA produced by the RiboMultiplier™ Sense-RNA Amplification Kit and unamplified total RNA with MAQC TaqMan® assays (~1,000 genes). Plots of log2 gene expression ratios for human brain and universal human reference total RNA obtained for ds cDNA synthesized from either RiboMultiplier sRNA (Panel A, X-axis) or unamplified total RNA (Panel B, X-axis) versus corresponding known MAQC TaqMan ratios (Y-axis) showed a similarly high correlation value (r=0.92), demonstrating excellent fidelity of the amplified sRNA compared to the unamplified total RNA.

스크린샷 2013-06-13 오후 3.02.15.png
  Table 1. 3´/5´ ratios for RiboMultiplier™ sRNA compared to aRNA produced by another RNA amplification procedure.Random-primed cDNA samples prepared from either RiboMultiplier™ sRNA or T7-dT aRNA were used in qPCR, and the 3´/5´ sequence ratios were determined for several genes. In general, 3´/5´ ratios around 1.0 were seen for the RiboMultiplier sRNA sample, demonstrating the enhanced 5´ sequence representation in sRNA as compared to T7-dT aRNA.

 스크린샷 2013-06-13 오후 3.02.31.png  Table 2. The RiboMultiplier™ sRNA Kit provides high yields of sRNA from as little as 10 ng of total RNA.

a. Approximately 2% of HeLa total RNA is poly(A) RN


[서울] T 02-2105-7020 / F 02-2105-7025 / E-mail info@philekorea.co.kr / 08507 서울 금천구 가산디지털1로 168, 우림라이온스밸리 A-606B
[대전] T 042-862-9636 / F 042-862-9638 / E-mail info@philekorea.co.kr / 34025 대전시 유성구 테크노2로 미건테크노월드 1차 201호


COPYRIGHT© 2013 PhileKorea. ALL RIGHTS RESERVED