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Reagent

Additional Reagent

Transformation & Storage Solution (TSS)

Applications
  • Preparation of E. coli competent cells for transformation.
Transformation & Storage Solution (TSS), developed at the U.S. National Institute of Health,1 enables researchers to prepare competent E. coli in a single step and to transform the cells without heat-shock. TSS has been reported to be faster and easier than other methods of producing competent cells, such as the traditional CaCl2 method described by Sambrook etal.2or other high-competency protocols.3

Transformation efficiencies depend on the strain of E. coli, as well as the nature and quality of the transforming DNA. For example, the transformation efficiencies observed for E. coli strains DH1, DH5α, HB101, JM109, LE392, MM294, SCS-1, and XL1-Blue ranged from 1.5 x 107 to 1.0 x 108/µg of DNA.

Benefits
  • Single-step preparation of competent cells.
  • Store prepared cells at -70°C with little or no loss in transformation efficiency.
  • Transform cells without heat-shock.
  • Transformation efficiencies of 106-108/µg DNA are typically obtained.




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Storage Buffer:
TSS is supplied as a ready-to-use 2X solution.

Quality Control: TSS is tested for transformation efficiency with appropriateE. coli strains.

References
  1. Chung, C.T. et al. (1989) Proc. Natl. Acad. Sci. USA 86, 2172.
  2. Sambrook, J. et al. (1989) in: Molecular Cloning: A Laboratory Manual (2nd ed.), Cold Spring Harbor Press, New York.
  3. Hondal, R. and Schaeffer, C. (1995) Epicentre Forum 2(3), 5.





Sterile Nuclease-Free Water

Epicentre's Molecular Biology Grade Sterile Nuclease-Free Water is free of DNA exo- and endonuclease activities, and RNase activity.



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