Transformation & Storage Solution (TSS)
- Preparation of E. coli competent cells for transformation.
Transformation efficiencies depend on the strain of E. coli, as well as the nature and quality of the transforming DNA. For example, the transformation efficiencies observed for E. coli strains DH1, DH5α, HB101, JM109, LE392, MM294, SCS-1, and XL1-Blue ranged from 1.5 x 107 to 1.0 x 108/µg of DNA.
Storage Buffer: TSS is supplied as a ready-to-use 2X solution.
Quality Control: TSS is tested for transformation efficiency with appropriateE. coli strains.
- Chung, C.T. et al. (1989) Proc. Natl. Acad. Sci. USA 86, 2172.
- Sambrook, J. et al. (1989) in: Molecular Cloning: A Laboratory Manual (2nd ed.), Cold Spring Harbor Press, New York.
- Hondal, R. and Schaeffer, C. (1995) Epicentre Forum 2(3), 5.
Sterile Nuclease-Free Water
Epicentre's Molecular Biology Grade Sterile Nuclease-Free Water is free of DNA exo- and endonuclease activities, and RNase activity.