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Genomic DNA
eCube Tissue DNA Mini Kit
eCube Tissue DNA Mini Kit
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eCube Tissue DNA Mini Kit

For isolation of total DNA from animal tissue, fixed tissue, bacteria, yeast or blood spot

eCube Tissue DNA Mini Kit is an excellent tool offering a speedy and economic method to purify total DNA from several types of animal tissue. Some specially modified protocols are developed for other samples, such as bacterial, fixed tissue, yeast and blood spot etc. This technology first lyses cells and degrades protein by using a chaotropic salt and Proteinase K, then binds DNA to silica based membranes, washes DNA with ethanol contained wash buffer and then elutes purified DNA by low salt elution buffer. Compare with other harmful and consuming procedures. 

The size of purified DNA is up to 50Kb (predominantly 20 - 30 Kb). DNA of this length performs the highest efficiency during PCR reaction. 

After using this kit, the high quality total DNA can be used directly for the down-stream applications.

스크린샷 2013-06-26 오후 3.52.14.png
The Quality of DNA after Purification Genomic DNA extracted from the indicated mouse tissue by the eCube Tissue DNA Mini Kit.
For each tissue, the amount of undigested (left) and EcoRI digested (right) are equivalent M1: 1 Kb DNA Ladder (100 bp - 12,000 bp)



Sample: 
Up to 25 mg of fresh frozen tissue

Expectant Yield:
Up to 50 μg of total DNA, depends on the sample types

Handing time: 
About 30 - 60 minutes

Protocols Included:
Tissue, Bacteria, Fixed Tissue, Yeast, Dried Blood Spot

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