eCube Plant DNA Mini Kit
For isolation of total DNA from plant tissue and cell.
eCube Plant DNA Mini Kit provides a fast and simple method to isolate total DNA (genomic DNA, mitochondrial and chloroplast) from plant tissue and cells. In the process, sample is distrusted by grinding in liquid nitrogen and lysis buffer incubation. The Lysate is treated with RNase A to degrade RNA and filtrated by filter column to remove cell debris and salt precipitations. In the presence of binding buffer with chaotropic salt, the genomic DNA in the lysate binds to glass fiber matrix in the spin column. The contamin are washed with an ethanol contained wash buffer and finally, the purified genomic DNA is eluted by low salt elution buffer or water.
The protocol does not require DNA phenol extraction and alcohol precipitation.
The purified genomic DNA is ready for PCR, real-time PCR, Southern blotting and RFLP.
The Quality of DNA After Purification Agarose gel (1.0%) analysis of DNA isolated from the indicated young leaves with eCube Plant DNA Mini Kit.
100 mg of each sample is used.
After DNA purification, 3 μl of 200 μl elution product is loaded in each well.
1: Cinnamomun camohora
2: Pisum sativum (Pea Sprout)
3: Arabidopsis thaliana
4: Oryze sativa (Rice)
5: Ipomose batatas (Sweet Potato)
6: Rhizoma dioscoreae (Chinese Yam)
7. Populus tremula (Aspen)
8. Flammulina velutipes
9: Oxalis comiculats (Fourleaf clover)
Typical yields of genomic DNA obtained from various plant samples