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1-Step
QuantiSpeed Probe One-Step Kit
QuantiSpeed Probe One-Step Kit
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QuantiSpeed Probe One-Step Kit

Optimized solution for real-time RT-PCR using SYBR Green I

QuantiSpeed Probe One-Step Kit uses the latest developments in reverse transcriptase technology and buffer chemistry for efficient cDNA synthesis and qPCR in a single tube. The qPCR is performed by Philekorea Hot Stable Taq DNA Polymerase, resulting in robust RT-PCR performance.

QuantiSpeed Probe One-Step Kit can be used to quantify any RNA template including mRNA, total RNA and viral sequences. Extremely low copy number targets can be detected specifically with high efficiency. Proprietary small molecular inhibitor technology prevents formation of primer dimers and non-specific products leading to improved reaction sensitivity and specificity. Combining the latest advancements in polymerase technology and advanced buffer chemistry we offer market leading performance with minimal or no optimization.

Many modern real-time PCR instruments have a fast cycling mode. The same developments that improve the sensitivity and consistency of 
QuantiSpeed Probe One-Step Kit in standard cycling conditions also allow for industry leading performance in fast and ultra fast cycling conditions.

Features
  • Thermostable reverse transcriptase 45°C to 55°C
  • Contains advanced RNase inhibitor
  • Rapid extension rate for early Ct values
  • Market leading sensitivity - increased limit of detection
  • Compatible on all real-time PCR platforms - standard and fast cycling conditions
Applied Biosystems  7500, 7500 FAST, StepOneTMStepOneTM Plus

Bio-Rad®  CFXTM

Illumina®  EcoTM

Qiagen/Corbett  Rotor-GeneTM 3000, 6000, Rotor-Gene TM Q

Roche Applied Science  Lightcycler®480, Lightcycler®Nano
 Cycles Temperature   Time  Notes
 1
45°C to 55°C 10 min
Reverse transcription, 45°C is recommended for most applications, 55°C 
should be used only when amplicon contains regions of high secondary 
structure
1 95°C 2 min Polymerase activation, 2 minutes for cDNA and 3 minutes for genomic
 40
95°C
60°C to 65°C
5 seconds
20-30 seconds
Denaturation
Anneal/Extension, do not exceed 30 seconds, do not use temperatures  below 60°C
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[Deajeon] MigunTechnoWorld 1-201, Techno2-ro, Yuseong-gu, Daejeon, Korea 34025 / TEL :+82-42-862-9636  /  FAX :+82-42-862-9638 / email : info@philekorea.co.kr