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QuantiSpeed SYBR (No/Low/HI)-ROX Kit
QuantiSpeed SYBR (No/Low/HI)-ROX Kit
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QuantiSpeed SYBR (No/Low/Hi)-ROX Kit

Optimized solution for Real Time PCR using SYBR

Philekorea use a proprietary intercalating dye which does not inhibit PCR, unlike other popular fluorescent dyes. Combined with advanced enzyme, hot-start and reaction buffer technology we offer market leading sensitivity and reproducibility.

QuantiSpeed SYBR Kit can be used to quantify any DNA template including genomic, cDNA and viral sequences. Extremely low copy number targets can be detected specifically with high efficiency. Proprietary small molecular inhibitor technology prevents formation of primer dimers and non-specific products leading to improved reaction sensitivity and specificity. 

Combining the latest advancements in polymerase technology and advanced buffer chemistry we offer market leading performance with minimal or no optimisation.

Many modern real-time PCR instruments have a fast cycling mode. The same developments that improve the sensitivity and consistency of QuantiSpeed SYBR Kit in standard cycling conditions also allow for industry leading performance in fast and ultra fast cycling conditions.

  • Accurate quantificatin : hot-start capability saves time and reduces primer-dimer formation.
  • Sensitive : from low copy targets.
  • Rapid : Unique buffer chemistry for hightest specificity and snesitivity
  • Flexible : compatible with all standard and fast cycling instruments

Applied Biosystems  7500, 7500 FAST, StepOneTMStepOneTM Plus

Bio-Rad®  CFXTM

Illumina®  EcoTM

Qiagen/Corbett  Rotor-GeneTM 3000, 6000, Rotor-Gene TM Q

Roche Applied Science  Lightcycler®480, Lightcycler®Nano
 Cycles Temperature   Time  Notes
2 min
Polymerase activation, 2 minutes for cDNA and 3 minutes for genomic
60°C to 65°C
5 seconds
20-30 seconds
Anneal/Extension, do not exceed 30 seconds, do not use temperatures  below 60°C
 Refer to insturment instructions
Optional melt profile analysis 

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