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QuantiSpeed Probe Kit
QuantiSpeed Probe Kit
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QuantiSpeed Probe Kit

Optimized solution for Real Time PCR using probe

QuantiSpeed Probe Kit is a universal probe kit designed for use in all probe based real-time PCR assays. Whether your application is for a singleplex or multiplex expression study, a genotyping experiment or a diagnostic assay, QuantiSpeed Probe Kit is the robust choice for all your probe based real-time PCR needs.

QuantiSpeed Probe Kit can be used to quantify any DNA template including genomic, cDNA and viral sequences. Extremely low copy number targets can be detected specifically with high efficiency.

Proprietary small molecular inhibitor technology prevents formation of primer-dimers and non-specific products leading to improve reaction sensitivity and specificity. Combining the latest advancements in polymerase technology and advanced buffer chemistry we offer market leading performance with minimal or no optimisation and high efficiency in multiplexed reactions.

Many modern real-time PCR machines have a fast cycling mode. The same developments that improve the sensitivity and consistency of QuantiSpeed Probe Kit in standard cycling conditions also allow for industry leading performance in fast and ultra fast cycling conditions.


Feature
  • Specificity : minimal non-specific activity
  • Sensitivity : perfect for low copy number samples
  • Speed : earlier Ct with fast protocols
  • Effecient multiplexing : no loss in efficiency using multiple probes
Applied Biosystems  7500, 7500 FAST, StepOneTMStepOneTM Plus

Bio-Rad®  CFXTM

Illumina®  EcoTM

Qiagen/Corbett  Rotor-GeneTM 3000, 6000, Rotor-Gene TM Q

Roche Applied Science  Lightcycler®480, Lightcycler®Nano
 Cycles Temperature   Time  Notes
 1
95°C 2 min Polymerase activation, 2 minutes for cDNA and 3 minutes for genomic
 40
95°C
60°C to 65°C
5 seconds
20-30 seconds
Denaturation
Anneal/Extension, do not exceed 30 seconds, do not use temperatures  below 60°C


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