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HRM
QuantiSpeed HRM Kit
QuantiSpeed HRM Kit
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QuantiSpeed HRM Kit

Optimized solution for High Resolution Melt Analysis

Combined with the latest advancements  in polymerase technology and advanced  buffer chemistry QuantiSpeed HRM Mix  offers market leading accuracy in High Resolution Melt (HRM) analysis. QuantiSpeed HRM Mix uses 3rd generation,  saturating intercalating dye which does  not inhibit PCR.  

HRM analysis is a powerful technique for the  analysis of mutations, polymorphisms and  epigenetic differences in double-stranded DNA  samples.

QuantiSpeed HRM Mix uses proprietary small molecular inhibitor technology that prevents  formation of primer-dimers to improve reaction  sensitivity and specificity.  

High-throughput screening has resulted in a  buffer system that allows efficient amplification  from GC-rich and AT-rich templates, under fast  and standard cycling conditions.

Applied Biosystems 7900HT FAST, StepOneTM Plus, 7500 FAST

Bio-Rad® CFX96TM, CFX384TM

Illumina® EcoTM

Corbett/QIAGEN Rotor-Gene 6000, Rotor-Gene Q

Roche Applied Science Lightcycler®480, Lightcycler®Nano
 Cycles
Temperature 
 Time
 Notes
 1
95°C
2 min
Polymerase activation, 2 minutes for cDNA and 3 minutes for genomic
 40
95°C
60°C to 65°C
5 seconds
20-30 seconds
Denaturation
Anneal/Extension, do not exceed 30 seconds, do not use temperatures  below 60°C
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