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QuantiSpeed HRM Kit

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Overview

Instrument compatibility

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QuantiSpeed HRM Kit

Optimized solution for High Resolution Melt Analysis

Combined with the latest advancements in polymerase technology and advanced buffer chemistry QuantiSpeed HRM Mix offers market leading accuracy in High Resolution Melt (HRM) analysis. QuantiSpeed HRM Mix uses 3rd generation, saturating intercalating dye which does not inhibit PCR.

HRM analysis is a powerful technique for the analysis of mutations, polymorphisms and epigenetic differences in double-stranded DNA samples.

QuantiSpeed HRM Mix uses proprietary small molecular inhibitor technology that prevents formation of primer-dimers to improve reaction sensitivity and specificity.

High-throughput screening has resulted in a buffer system that allows efficient amplification from GC-rich and AT-rich templates, under fast and standard cycling conditions.

Overview

Instrument compatibility

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Applied Biosystems 7900HT FAST, StepOne^TM Plus, 7500 FAST

Bio-Rad^® CFX96^TM, CFX384^TM

Illumina^® Eco^TM

Corbett/QIAGEN Rotor-Gene 6000, Rotor-Gene Q

Roche Applied Science Lightcycler^®480, Lightcycler^®Nano

Overview

Instrument compatibility

Program the Instruments

Cycles	Temperature	Time	Notes
1	95°C	2 min	Polymerase activation, 2 minutes for cDNA and 3 minutes for genomic
40	95°C 60°C to 65°C	5 seconds 20-30 seconds	Denaturation Anneal/Extension, do not exceed 30 seconds, do not use temperatures below 60°C