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유전자 분석장비 SuperCycler Nanostring nCounter System POCKIT Smart PCR System UV-VIS Spectrophotometer/Fluorometer Mic Real-Time PCR nanoString GeoMx™ Digital Spatial Profiler Liquid Handling System : myra 이미지 분석장비 Enduro Gel-Doc Enduro GDS Touch TissueGnostics Azure Imaging System Sapphire Biomolecular Imager 계수기 Moxi Z Mini Automated Cell Counter Moxi Go II CellDrop™ Cell Counters Moxi GO 532 유전자 증폭장비 SuperCycler Qamp mini Microplate reader Ao Absorbance Microplate Reader Byonoy Absorbance 96 LSPR 분석기 Instant NanoBiosensors INB-D200 Biomarker Analyzer	추출장비 taco Aurora System Semi-Automated Nucleic Acid Extraction System taco Mini Automatic Nucleic Acid Extraction System iColumn Extraction System iColumn LV System 자동화 장비 LabTurbo 24 BlotCycler™ LabTurbo96XC LabTurbo 48 LabTurbo 24 AIO SP-qPCR 조직파쇄기 tacoPrep Bead beater 세포배양장비 CLINOSTAR ™	소형원심분리기 Multi-Well Plate Centrifuge 12 strip tube Centrifuge SmartFuge Centrifuge 전기영동장치 Protein Electrophoresis System Blotting System Aqua™ Vertical Gel Running System 자동전기영동장치 Qsep1 Qsep1-Lite Qsep100 Qsep100 Advance Qsep400 vortex MTV-1 mini vortex Lab instrument LED Transilluminator Rotary Mixing Solution CO-01 Tube Roller CO-02/CO-02S Vacuum Pump CO-03 / CO-03S Mini Stirring Magnetic (Cute Stir) CO-12 Digital Magnetic Stirrer Hot Plate Series CO-04/CO-05/CO-05S Glass Beads Sterilizer CO-06 Handheld Homogenizer CO-07 Handheld UV Analyzer CO-08 Black-box Type UV Analyzer CO-09 Mupid Mini-EP Mini Electrophoresis System	PCR miniPCR® thermal cycler Electrophoresis and visualization Electrophoresis with built-in transilluminator blueBox™ Transilluminator with Imaging Hood Other Hardware Set of micropipettes Series Gyro™ microcentrifuge Bundle Set miniPCR® Lab in a Box™ Kit Seriese miniPCR® Pack Series
증폭 및 분석장비	추출 및 분주장비	기초 및 일반장비	minipcr bio

Fluorescent Western Blotting MultiColor Fluorescent Secondary Antibodies Streptavidin Conjugates FLASHBlot Transfer Buffer Chemiluminescent Western Blotting WesternBright ECL & ECL Spray HRP Conjugates Quantum HRP Substrate Sirius HRP Substrate WesternBright ChemiPen ELISA HRP Conjugates ELISABright Electrophoresis Protein sample loading buffers Scarlet protein stain Purification SDS-PAGE sample preparation kit RapidClean Buffers and Solutions blocking solution Washing solution Avant buffer pouches LucentBlue X-ray film Background quenching sheets Transfer membranes Incubation trays Blotting papers Visio	Array-Based Analysis RNA Amplification for qPCR RNA Labeling RNA Labeling for Expression Microarrarys End-Point PCR & RT End-Point PCR Kits cDNA Synthesis dNTP Solutions Nucleic Acid Purification DNA Extraction RNA Extraction DNA Purification. Genomic DNA Purification. Cloned RNA Purification Lysozymes In Vitro Transcription In Vitro Transcription Kits NTP Solutions Library Construction Vector and Insert Preparation Cloning, cDNA Cloning, Genetics Competent Cells Additional Reagents Transposomics™ In Vivo Transposomics™ In Vitro Transposomics™ RNA Polyadenylation RNA Polyadenylation	Accutase® Cell Detachment Solution- 100ml Instant-Bands Phytotech PhytoTech LABS 식물 자재 Ginrei Lab Interactive Co-Culture Plate (ICCP) Synthego Engineered Cells DeNovix Fluorescence Quantification Assay Kit transOMIC technologies Innovative Genomic Tools
Advansta	Epicentre	기타제품

Cellular Analysis Cloning & Synthetic Biology DNA Amplification, PCR & qPCR Genome Editing RNA Analysis Sample Preparation for Next Generation Sequencing Epigenetics Protein Expression & Purification Protein Analysis & Tools Glycobiology & Proteomics	Restriction Endonucleases PCR, qPCR & Amplification Technologies DNA Modifying Enzymes Sample Preparation for Next Generation Sequencing Nucleic Acid Purification Markers & Ladders RNA Reagents DNA Assembly, Cloning and Mutagenesis Kits Genome Editing Gene Expression Cellular Analysis Epigenetics Protein Expression & Purification Technologies Competent Cells Protein Tools Glycobiology DNA Plasmids & Substrates Buffers Strains Discontinued	NEB 제품 견적 및 주문 문의 NEB 샘플 신청 NEB Info.	NEBNext Direct® Cancer HotSpot Panel NEBNext® rRNA Depletion Kit NEBNext® Ultra™ II RNA Library Prep Kit NEBNext® Microbiome DNA Enrichment Kit NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® NEBNext Direct® BRCA1BRCA2 Panel NEBNext® small RNA Library Prep Kit
Applications	Product Catalog	NEB 제품 견적 및 주문	NEBNext - NGS Kit

Genomic DNA eCube Tissue DNA Mini Kit eCube Plant DNA Mini Kit eCube Stool DNA Mini Kit eCube Soil DNA Mini Kit eCube Blood DNA Mini Kit RNA eCube Blood RNA Mini Kit eCube Tissue RNA Mini Kit eCube Plant RNA Mini Kit eCube-Zol RNA Reagent eCube-RNA Stabilization Solutions Virus NA eCube Viral Nucleic Acid Mini Kit Plasmid eCube Plasmid DNA Mini Kit eCube Plasmid DNA Midi/Maxi Kit Gel & PCR purification eCube PCR Purification Kit eCube Gel&PCR Purification Kit eCube Gel Purification Kit	1-Step QuantiSpeed SYBR (No/Low/Hi)-ROX One-Step Kit QuantiSpeed Probe (No/Low/Hi)-ROX One-Step Kit 2-Step QuantiSpeed Probe (No/Low/Hi)-ROX Kit QuantiSpeed SYBR (No/Low/HI)-ROX Kit HRM QuantiSpeed HRM Kit	PCR & RT Enzyme 2x Hot Taq Master Mix 2x Taq Master Mix Taq DNA Polymerase Hot Taq DNA Polymerase 2x pfu Master Mix cDNA Synthesis Kit One-step RT-PCR Kit	DNase DNase I (1g) RNase RNase Inhibitor RNase A Powder RNase A Solution Agarose Agarose dNTP Solution dNTP Solution Proteinase K Proteinase K Powder Proteinase K Solution DNA Loading Dye DNA Loading Dye DNA Ladder DNA Ladder	Tube 1.5mL Screw Tube, skirted base, ribbed, Clear 1.5mL Screw Tube, skirted base, ribbed, amber 2.0mL Screw Tube, skirted base, Graduated, writing area, amber 2.0mL Assembled Screw Tube, skirted base, Graduated, writing area, clear, Sterile Combo Pack 12-strip PCR Tubes & Dome Caps, Clear 0.5mL Micro tube,Clear(natural) 2.0 mL Graduated Microcentrifuge Tube, Clear 5 mL Microcentrifuge Tube, Clear 0.2mL Combo Pack 8-Strip PCR Tubes & Flat Caps, Clear(NAT) 0.2mL PCR Tube, Attached Dome Cap, NAT 0.2mL PCR Tube, Attached Flat Cap, Natural 0.2mL 8-Strip Tubes with Individually-Attached Dome Caps, Clear 0.2mL SnapStrip PCR Tubes,8-strips,Flat Caps, Clear 1.8 mL Internally threaded CryoFreeze vial, Stargrip base, Sterile 1.8 mL Externally threaded CryoFreeze vial, Stargrip base, Sterile 1.2 mL 8-STP TUBE RK Well & Rack 96-Well PCR Plate, Flat Top, Standard, Clear 96-Well PCR Plate, Low-Profile, Non-Skirted, Clear 96-Well PCR plate, full-skirted 96-Well Semi Skirt PCR P96-WELL PLATE, NAT Semi Skirt, Low Profile, 96-Well Light Cycler PCR Plate, White IsoFreeze MCT,Pink IsoFreeze PCR Pink IsoFreeze PCR Yel [96tubes x 2rk] IsoFreeze PCR SBS-format Rack, Pink IsoFreeze PCR SBS-format Rack, Yellow [96tubes x 2rk] Tip & Cap Screw Cap(with O-ring) 4colors
Purification	Real Time PCR Enzyme	PCR & RT Enzyme	Related Product	Laboratory Supplies

nCounter 서비스	Real Time PCR 서비스
nCounter 서비스	Real Time PCR 서비스

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Instruments

Reagents

NEB

PK Brand

eCube Tissue RNA Mini Kit

For purification of total RNA from animal tissue and cultured cells.

The eCube Tissue RNA Mini Kit is designed for extraction of total RNA from animal tissue and cultured cells. Some specially modified protocols are developed for other samples, such as bacteria and yeast. This method first lyses cells by using a chaotropic salt , then binds RNA to silica-based membranes, washes RNA with ethanol-contained wash buffer and then elutes purified RNA by RNase-free water.

The pyrified RNA is ready for RT-PCR, real-time PCR, northern blotting, primer extension and cDNA library construction.

Agarose gel of total RNA purified 5 μl of elution product from 10 mg of different tissues was loaded to each lane.
All tissues were from a mouse.

1. Brain
2. Lung
3. Liver
4. Spleen
5. Kidney

Sample amount and yield

Overview

Specification

Download

Sample:
up to 30 mg animal tissue
Up to 1 x 10⁷ animal cultured cells, 1 x 10⁹ bacterial cells,or 5 x 10⁷ yeast

Expectant Yield:
up to 40 μg total RNA

Handing time:

About 30 minutes

Overview

Specification

Download

eCube Tissue RNA Mini Kit.pdf

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