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eCube Tissue RNA Mini Kit

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Overview

Specification

eCube Tissue RNA Mini Kit

For purification of total RNA from animal tissue and cultured cells.

The eCube Tissue RNA Mini Kit is designed for extraction of total RNA from animal tissue and cultured cells. Some specially modified protocols are developed for other samples, such as bacteria and yeast. This method first lyses cells by using a chaotropic salt , then binds RNA to silica-based membranes, washes RNA with ethanol-contained wash buffer and then elutes purified RNA by RNase-free water.

The pyrified RNA is ready for RT-PCR, real-time PCR, northern blotting, primer extension and cDNA library construction.

Agarose gel of total RNA purified 5 μl of elution product from 10 mg of different tissues was loaded to each lane.
All tissues were from a mouse.

1. Brain
2. Lung
3. Liver
4. Spleen
5. Kidney

Sample amount and yield

Overview

Specification

Sample:
up to 30 mg animal tissue
Up to 1 x 10⁷ animal cultured cells, 1 x 10⁹ bacterial cells,or 5 x 10⁷ yeast

Expectant Yield:
up to 40 μg total RNA

Handing time:

About 30 minutes

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