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Genomic DNA eCube Tissue DNA Mini Kit eCube Plant DNA Mini Kit eCube Stool DNA Mini Kit eCube Soil DNA Mini Kit eCube Blood DNA Mini Kit RNA eCube Blood RNA Mini Kit eCube Tissue RNA Mini Kit eCube Plant RNA Mini Kit eCube RNA Clean-up Kit eCube-Zol RNA Reagent eCube-RNA Stabilization Solutions Virus NA eCube Viral Nucleic Acid Mini Kit Plasmid eCube Plasmid DNA Mini Kit eCube Plasmid DNA Midi/Maxi Kit Gel & PCR purification eCube PCR Purification Kit eCube Gel&PCR Purification Kit eCube Gel Purification Kit	1-Step QuantiSpeed Probe One-Step Kit QuantiSpeed SYBR One-Step Kit 2-Step QuantiSpeed Probe Kit QuantiSpeed SYBR Green Kit HRM QuantiSpeed HRM Kit QuantiSpeed Genotyping Kit	제품 준비중	RNase Inhibitor Agarose Agarose dNTP dNTP Solutions DNA ladder & Loading dye DNA Loading Dye DNA Ladder
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Instruments

시약류

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실험 대행 서비스

RNA

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eCube Tissue RNA Mini Kit

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Overview

Specification

eCube Tissue RNA Mini Kit

For purification of total RNA from animal tissue and cultured cells.

The eCube Tissue RNA Mini Kit is designed for extraction of total RNA from animal tissue and cultured cells. Some specially modified protocols are developed for other samples, such as bacteria and yeast. This method first lyses cells by using a chaotropic salt , then binds RNA to silica-based membranes, washes RNA with ethanol-contained wash buffer and then elutes purified RNA by RNase-free water.

The pyrified RNA is ready for RT-PCR, real-time PCR, northern blotting, primer extension and cDNA library construction.

Agarose gel of total RNA purified 5 μl of elution product from 10 mg of different tissues was loaded to each lane.
All tissues were from a mouse.

1. Brain
2. Lung
3. Liver
4. Spleen
5. Kidney

Sample amount and yield

Overview

Specification

Sample:
up to 30 mg animal tissue
Up to 1 x 10⁷ animal cultured cells, 1 x 10⁹ bacterial cells,or 5 x 10⁷ yeast

Expectant Yield:
up to 40 μg total RNA

Handing time:

About 30 minutes

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