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RNA
eCube Plant RNA Mini Kit
eCube Plant RNA Mini Kit
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eCube Plant RNA Mini Kit

For purification of total RNA from plant tissue and cells

The eCube Plant RNA Mini Kit is specially designed for purification of total RNA from a variety of plant tissues. The method uses detergents and a chaotropic salt to lyse cell and inactivate RNase. In the presence of binding buffer with chaotropic salt, the total RNA in the lysate binds to glass fiber matrix in the spin column. The contaminants are washed with an ethanol contained wash buffer.

Finally, the purified total RNA is eluted by RNase-free water. The protocol does not require phenol extraction and alcohol precipitation. 

The purified total RNA is ready for RT, RT-PCR, real-time PCR, Northern blotting. ssRNA and dsRNA of 200 bp to 1000’s of bps in length are efficiently purified.

Procedure
스크린샷 2013-06-26 오후 5.37.09.png

Sample:
up to 100 mg plant tissue or 1 X 107  plant cells

Expectant Yield:
up to 5 - 30 μg total RNA from young leave

Handing time: 
About 30 - 60 minutes


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