eCube Plant RNA Mini Kit
For purification of total RNA from plant tissue and cells
The eCube Plant RNA Mini Kit is specially designed for purification of total RNA from a variety of plant tissues. The method uses detergents and a chaotropic salt to lyse cell and inactivate RNase. In the presence of binding buffer with chaotropic salt, the total RNA in the lysate binds to glass fiber matrix in the spin column. The contaminants are washed with an ethanol contained wash buffer.
Finally, the purified total RNA is eluted by RNase-free water. The protocol does not require phenol extraction and alcohol precipitation.
The purified total RNA is ready for RT, RT-PCR, real-time PCR, Northern blotting. ssRNA and dsRNA of 200 bp to 1000’s of bps in length are efficiently purified.