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eCube Plasmid DNA Mini Kit
eCube Plasmid DNA Mini Kit
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eCube Plasmid Mini Kit

For purification of up to 30ug plasmid DNA from bacterial culture.

The eCube Plasmid DNA Mini Kit provides a rapid, phenol-free method for the extraction of high-purity plasmid DNA from bacterial cultures such as E.coli, which bacteria is pellet, lysed, and then neutralized.
 
The extracted DNA can be used in a variety of applications such as PCR, cloning, sequencing, in vitro transcription, and labeling. Also, as a column-type tube is utilized in the purification process, extraction is carried out in three simple steps of binding/washing/elution. Once bound, the DNA is washed and then
eluted from the column, ready for use.

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DNA Sequencing Analysis

Sequencing of plasmid purified with eCube Plasmid DNA Mini Kit.
The sequence was analyzed with Big- Dye Terminator chemistry on ABI 3700

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Agarose Gel Analysis Of Plasmids Purified Restriction analysis of 4 plasmids extracted by the eCube Plasmid DNA Mini Kit.

1: pBluescript II
2. pUC 18
3: pBR 322
4: pGem with 1 Kb insert
5: pET 43.1 with 2.7 Kb insert
6: cosmid (50 Kb)
M1: 1 Kb DNA Ladder
M2: Lambda-HindIII 


Sample: 
1 - 5 ml overnight culture

Plasmid Size: 
< 12 Kb

Expectant Yield:
20 - 30 μg of high-copy plasmid
3 - 10 μg for low-copy plasmid

Handing time: 
about 25 min

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