eCube Plasmid Mini Kit
For purification of up to 30ug plasmid DNA from bacterial culture.
The eCube Plasmid DNA Mini Kit provides a rapid, phenol-free method for the extraction of high-purity plasmid DNA from bacterial cultures such as E.coli, which bacteria is pellet, lysed, and then neutralized.
The extracted DNA can be used in a variety of applications such as PCR, cloning, sequencing, in vitro transcription, and labeling. Also, as a column-type tube is utilized in the purification process, extraction is carried out in three simple steps of binding/washing/elution. Once bound, the DNA is washed and then
eluted from the column, ready for use.
The extracted DNA can be used in a variety of applications such as PCR, cloning, sequencing, in vitro transcription, and labeling. Also, as a column-type tube is utilized in the purification process, extraction is carried out in three simple steps of binding/washing/elution. Once bound, the DNA is washed and then
eluted from the column, ready for use.
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DNA Sequencing Analysis
Sequencing of plasmid purified with eCube Plasmid DNA Mini Kit. The sequence was analyzed with Big- Dye Terminator chemistry on ABI 3700 |
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Agarose Gel Analysis Of Plasmids Purified Restriction analysis of 4 plasmids extracted by the eCube Plasmid DNA Mini Kit.
1: pBluescript II
2. pUC 18 3: pBR 322 4: pGem with 1 Kb insert 5: pET 43.1 with 2.7 Kb insert 6: cosmid (50 Kb) M1: 1 Kb DNA Ladder M2: Lambda-HindIII |