eCube Plasmid DNA Midi/Maxi Kit
For purification of up to 650 ug high quality plasmid DNA from bacterial culture.
The eCube Plasmid Midi and Maxi Kit provides pre-packed an ion exchange resin column for high-purity plasmid DNA. This kit provide the alkaline lysis reagents and the columns packed with anion-exchanger resin. After the cells lysis, the plasmid DNA is bound to the resin insided the column by a gravity-flow procedure, and the contaminants can be remove with wash buffer. The purity of purified plasmid DNA is equity to that obtained by 2x CsCl-gradient centrifugation and suitable for all molecular biological applications such as transfection, in vitro transcription and translation, and all enzymatic modification.
Agarose Gel Analysis Of Plasmids
Purified Agarose gel analysis of various plasmid DNA purified with Midi/Maxi Kit. Plasmid pBluescript (high-copy) and pBR322 (low-copy) are purified with the Plasmid DNA Midi / Maxi Kit. DNA in crude lysate from alkaline lysis and fractions from each gravity-flow step was collected by isopropanol precipitation and load to each lane.
C: crude lysate
M: 1 Kb DNA Ladder