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eCube Plasmid DNA Midi/Maxi Kit
eCube Plasmid DNA Midi/Maxi Kit
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eCube Plasmid DNA Midi/Maxi Kit

For purification of up to 650 ug high quality plasmid DNA from bacterial culture.

The eCube Plasmid Midi and Maxi Kit provides pre-packed an ion exchange resin column for high-purity plasmid DNA. This kit provide the alkaline lysis reagents and the columns packed with anion-exchanger resin. After the cells lysis, the plasmid DNA is bound to the resin insided the column by a gravity-flow procedure, and the contaminants can be remove with wash buffer. The purity of purified plasmid DNA is equity to that obtained by 2x CsCl-gradient centrifugation and suitable for all molecular biological applications such as transfection, in vitro transcription and translation, and all enzymatic modification.

High-copy              Low-copy
Plasmid                   Plasmid

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Agarose Gel Analysis Of Plasmids

Purified Agarose gel analysis of various plasmid DNA purified with Midi/Maxi Kit. Plasmid pBluescript (high-copy) and pBR322 (low-copy) are purified with the Plasmid DNA Midi / Maxi Kit. DNA in crude lysate from alkaline lysis and fractions from each gravity-flow step was collected by isopropanol precipitation and load to each lane.
C: crude lysate
F: flow-ghrough
W: wash
E: elute
M: 1 Kb DNA Ladder


Procedure
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 Midi Kit
Sample:
up to 60 ml for high-copy number plasmid
up to 120 ml for low-copy number plasmid

Binding Capacity: 
up to 650 μg of high-copy plasmid


 Maxi Kit
Sample:
up to 60 - 240 ml for high-copy number plasmid
up to 200 - 480 ml for low-copy number plasmid

Binding Capacity: 
up to 1.5 mg of high-copy plasmid
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