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Aurora System
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Aurora System

Nucleic Acid Purification and Concentration System

Boreal’s nucleic acid purification instruments are based on SCODA (Synchronous Coefficient of Drag Alteration) a novel, proprietary electrophoresis technology that selectively concentrates nucleic acids from large sample volumes to a small, concentrated amount of gel or buffer.
SCODA electrophoretic purification is fundamentally different from other extraction methods, with significant advantages:
  • Unparalleled nucleic acid purity from the most difficult samples.
  • High yield from dilute or trace samples, enabling recovery where all other methods fail.
  • Exceptional removal of all PCR inhibitors including humic substances, independent of species.
  • Minimal sample handling enabling a simplifed workflow.
  • Concentration and purification of high molecular weight DNA 50 kb – 1Mb.

How it works:

Purification and concentration is accomplished by means of rotating electric fields that act uniquely on long, charged polymers, leaving other molecules unaffected. The electric field parameters can be adjusted to select very specifically for nucleic acids, rejecting contaminants and PCR inhibitors that, in affinity-based purification methods, often co-elute with DNA and RNA.
This method allows for very simple nucleic acid purification on our Aurora system: a sample up to 5 mL in volume that has undergone one of our prescribed lysis protocols is added to an input chamber on the disposable cartridge adjacent to concentration gel. The cartridge is inserted into the instrument and, following automated injection and concentration, the purified product is recovered by pipetting a small volume of buffer from the central reservoir.


Analyze your most challenging samples

Boreal’s electrophoretic extraction technology is proven to purify DNA and RNA from extremely low abundance and heavily inhibited samples including soils from the Atacama desert, Antarctic tundra, sea sediments, oil sands and stool.  Aurora also excels at extraction of high molecular weight DNA – see the product specification to learn more.



 Workflow

The Aurora workflow includes the following steps:
  1. Preparation of raw samples
  2. Lysis using chemical or mechanical methods or pre-processing
  3. Concentration and purification on the instrument
See the Applications page for specific sample protocols.








Cartridges

New: Aurora now accepts reusable agarose gel cartridges to minimize per-sample purification costs.  The refillable cartridges are provided with gel casting accessories and can be reused many times.  Additional cartridges and replacement parts are provided under the service contract providing a cost-effective solution for nucleic acid purification from the most challenging environmental samples.

Applications

Aurora purifies and concentrates nucleic acids from a wide range of samples:


The complete Aurora System comprises the instrument, an external chiller, and laptop and provided by Boreal Genomics.

Instrument
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Chiller
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Laptop
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Cartridge
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Instrument Performance
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Recent publications featuring Aurora

S. Schmedes et. al. | December 2012 | Int’l Journal of Legal Medicine
Effective removal of co-purified inhibitors from extracted DNA samples using synchronous coefficient of drag alteration (SCODA) technology | HTML

J. J. Schwartz et. al. | October 2012 | PNAS
Capturing native long-range contiguity by in situ library construction and optical sequencing | HTML

R. L. Welch et. al. | July 2012 | Lab on a Chip
Denaturation mapping of Saccharomyces cerevisiae | HTML

K. Engel et. al. | Jan 2012 | Journal of Microbiological Methods
Nonlinear electrophoresis for purification of soil DNA for metagenomics |HTML

C. Neish et. al. | ABSCICON 2012
A new approach for DNA detection in Mars analogue soils using SCODA |Poster

S. Schmedes et. al. | University of North Texas Health Sciences Center
The application of SCODA technology towards the extraction and purification of DNA from challenging forensic and medically-relevant samples | Poster

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