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Fragment Analzyer
Automated Capillary Electrophoresis System
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DNF‐900‐K0500 dsDNA Reagent Kit, 35‐500 bp 500 samples 로그인 확인 문의
DNF‐910‐K0500 dsDNA Reagent Kit, 35‐1,500 bp 500 samples 로그인 확인 문의
DNF‐910/15L80MK0500 dsDNA Reagent Kit, 35‐5,000 bp 500 samples 로그인 확인 문의
DNF‐920‐K0500 dsDNA Reagent Kit, 75‐15,000 bp 500 samples 로그인 확인 문의
DNF‐930‐K0500 dsDNA Reagent Kit, 75‐20,000 bp 500 samples 로그인 확인 문의
DNF‐479‐0500 Standard Sensitivity NGS Fragment Analysis Kit 500 samples 로그인 확인 문의
DNF‐486‐0500 High Sensitivity NGS Fragment Analysis Kit 500 samples 로그인 확인 문의
DNF‐492‐0500 Standard Sensitivity Large NGS Fragment Analysis Kit 500 samples 로그인 확인 문의
DNF‐493‐0500 High Sensitivity Large NGS Fragment Analysis Kit 500 samples 로그인 확인 문의
DNF‐489‐0500 Standard Sensitivity RNA Analysis Kit 500 samples 로그인 확인 문의
DNF‐491‐0500 High Sensitivity RNA Analysis Kit 500 samples 로그인 확인 문의
DNF‐487‐0500 Standard Sensitivity Genomic DNA Analysis Kit 500 samples 로그인 확인 문의
DNF‐488‐0500 High Sensitivity Genomic DNA Analysis Kit 500 samples 로그인 확인 문의
A2300‐1250‐5580 12‐Capillary Array Cartridge 50 um (ID) 55 cm (EFF), 80 cm (TOT), 2mL (RES 로그인 확인 문의
A2300‐1250‐3355 12‐Capillary Array Cartridge 50 um (ID) 33 cm (EFF), 55 cm (TOT), 2mL (RES) 로그인 확인 문의
A2300‐9650‐5580 96‐Capillary Array Cartridge 50 um (ID) 55 cm (EFF), 80 cm (TOT), 10mL (RES) 로그인 확인 문의
A2300‐9650‐3355 96‐Capillary Array Cartridge 50 um (ID) 33 cm (EFF), 55 cm (TOT), 10mL (RES) 로그인 확인 문의

Fragment AnalyzerTM

Automated Capillary Electrophoresis System

The Fragment Analyzer™ Automated CE System is a fluorescence-based capillary electrophoresis instrument for both sizing and quantifying nucleic acids (DNA and RNA). By using a sensitive intercalating dye coupled with a powerful LED light source, Fragment Analyzer™ Automated CE System obviates the need for fluorescent labeled primers and can be used to separate dsDNA fragments and RNA (mRNA and total RNA). The Fragment Analyzer™ Automated CE System is the most flexible system on the market today, with the greatest sensitivity, the highest separation resolution over a wide range, the widest dynamic range and the fastest separation times.
This instrument comes available for customers who wish to run either 12 samples or 96 samples at a time.
Currently kits are available to:
• quantify and qualify NGS fragments.
• quantify and qualify RNA.
• quantify and qualify genomic DNA.
• do Mutation Detection, also known as TILLiNG, a reverse genetics technique.
The application kits help laboratories to streamline workflow and avoid bottlenecks typically found with competitive lab-on-a-chip methods. Gels formulated to provide high resolution separation are also available for general fragment analysis, such as SSR/microsatellites, small and large PCR fragments (amplicons) and resolve fragments sized between 10 bp and 40,000 bp with resolution down to 2bp.
Each and every kit is performance-tested prior to shipment to ensure quality, accuracy and ease of use for customers. Most kits require a single pipetting step before placement of the sample plate onto the instrument.
An analytical software package, PROSize™, greatly reduces post-separation analysis time. Put them all together — the instrument itself, the QC-tested application kits, and the analytical software — and the combination makes the Fragment Analyzer™ Automated CE System a powerful package for reducing labor and improving lab workflow and throughput.


Contact us today and request an instrument for your free one day trial.


Parallel Capillary Electrophoresis: Description of Technology

Advanced Analytical's parallel capillary electrophoresis (CE) technology enables electro-driven analytical separations of DNA and RNA with on-line, simultaneous LED-based fluorescent detection.
  • exchangeable capillary array assembly.
  • fixed optical detection platform.
  • high-voltage power supply.
  • fluid pumping system.
  • automatic tray positioning system.
Customized software is tailored for specific applications and provides instrument control, data acquisition, and data analysis. End-users may view the CE separations as electropherograms, or as digital gel images.
The instrument is designed to handle either a 12 capillary array which can access all wells of a 96-well plate, 12 samples at a time or a 96 capillary array, able to sample all positions in one injection. Standard capillary dimensions are 50μm inner diameter (i.d.) and 200μm outer diameter (o.d.).
Anyone who was worked with agarose or acrylamide gel electrophoresis knows about the trade- off between speed vs. resolution. If you want high resolution; you use a long box. If you want answers quickly, you use a short box. Likewise with the Fragment Analyzer™ Automated CE System: various capillary lengths can be used, depending on the application, required resolution and desired speed of analysis. Longer arrays provide resolution down to 2 bp for fragments under 300 bp in length. Shorter arrays still provide good resolution with run times as fast as 15 minutes.
The inlet side of the capillary array is arranged in an 8 x 12 format with electrodes placed side-by-side with each capillary, allowing for direct injection via vacuum or voltage from standard 96-well plates. The capillaries are aligned in parallel in the detection window, which is positioned between the LED light source and the detector assembly. The outlets of the capillaries are bundled to a common reservoir where they are connected to a ground. The capillary arrays are modular in nature and a new capillary array can be exchanged in less than five minutes.
Room temperature air is circulated around the capillaries to modulate the temperature during the CE separation. Technology Schematic (12 capillary Version shown)
FS12Diagram_v2C.jpg



Maximum Sample Throughput (12 capillary array):
  • 12 samples in 30 minutes for NGS fragments
  • 12 samples in 45 minutes for RNA
  • 12 samples in 12 minutes for DNA fragments
Maximum Sample Throughput (96 capillary array):
  • 96 samples in 60 minutes for NGS fragments
  • 96 samples in 70 minutes for RNA
  • 96 samples in 20 minutes for DNA fragments
Sample per run:
  • Up to 96
Maximum unattended sample capacity:
  • 288 samples
Minimum sample volume:
  • 20 µl; Typically 2µl of sample is required for dilution
Gel sizing ranges:
  • NGS; 35 - 6,000 bp
  • Total RNA and mRNA; 19 - 6,000 base
  • Genomic DNA
Resolution:
  • Gel dependent, as low as 2 bp
Sizing accuracy:
  • Typically 5% or better
Sensitivity:
  • <100 pg/µl for smears
Light source power:
  • One 1,000mW LED
LED wavelength:
  • 470nm
Emission collection devise:
  • CCD detector
Software:
  • Fragment Analyzer™
  • system software and PROSize™ data analysis software
Data export format:
  • Microsoft© Excel spreadsheet
Environmental conditions:
  • Indoor use, normal laboratory environment 20 - 23°C
Relative humidity range:
  • <80% (non-condensing)
Electrical:
  • 100 - 200 VAC; 50 - 60 Hz; 15A
Instrument dimensions:
  • Fully configured requires 61 cm H x 35 cm W x 51 cm D (24 x 14 x 20 in)
Instrument weight:
  • 38.5Kgs (85lbs)
Microsatellites/SSR

Microsatellite (SSR) analysis by PCR is a powerful molecular tool for screening organisms and determining polymorphic loci and genetic profiles. The Fragment Analyzer™ Automated CE System is an equally powerful and flexible fluorescence-based capillary electrophoresis instrument.
It provides size and quantification information for microsatellites and DNA fragments generated using unlabeled PCR primers specific to polymorphic microsatellite regions. With the highest resolution and fastest run times of any dsDNA fragment analysis capillary electrophoresis system, the Fragment Analyzer™Automated CE System improves laboratory throughput and reduces analysis time of microsatellites. Available in either 12-capillary or 96-capillary format.


Genomic DNA Analysis

Genomic DNA (gDNA) extraction from tissues, cells or organisms is a critical first step for all subsequent DNA analyses. Until now, assessing the quantity and quality of genomic DNA has typically required the use of two separate instruments: one for quantity, one for quality.
With the Fragment Analyzer™ Automated CE System platform, both genomic DNA quality and genomic DNA quantity can be assessed at the same time, using the same aliquot of sample and in less time than to do both by traditional methods.

Large Fragment Separation
AATI_Slide5_v1.png
Human gDNA sample analyzed with AATI’s high sensitivity gDNA kit. Peak is annotated by size. Measured concentration agreed to within 5% of fluorometric assay.


RNA Analysis

RNA research is the new frontier of discovery in life science. Labs that once analyzed dozens of RNA samples a week may now analyze hundreds or even thousands.
Assessment of RNA quality and RNA quantitation is critical for many molecular biology techniques. Manual assessment methods for both quantity and quality can use large amounts of precious materials and take a considerable amount of time to complete.
Several analytical instruments exist for the evaluation of RNA. However, these instruments either lack acceptable detection sensitivity or require significant hands-on time to complete chip loading and preparation. The Fragment Analyzer™ Automated CE System process for RNA – either for total RNA and/or messenger RNA – is completely automated, requiring minimal hands on time.
The Fragment Analyzer™ Automated CE System provides an RQN measurement to assist with RNA analysis.RQN stands for RNA Quality Number. Derived from PROSize® analytical software, RQN assesses the quality or integrity of total RNA on a scale of 1-to-10, and allows for correlation to legacy data.

scrabble_tiles.jpg

NGS Fragment Analysis

Accurately quantifying and qualifying Next Gen Sequencing (NGS) fragments in a high throughput automated fashion is becoming critically important for more laboratories. The labs are seeing increased demand from researchers who want to sequence smaller amounts of information, but from larger pools of organisms.

Typical NGS Separation
AATI_NGSSlideA_v1.png
Table shows quantitative information from NGS sample library preparation prior to running on Next Gen Sequencer. Electropherogram overlay plot shows size distribution of the NGS libraries samples, along with a sizing ladder (X-axis).

Overlap of NGS Libraries

AATI_NGSSlideB_v1.png
Different-sized NGS libraries from various fragmentation methods, such as enzymatic, sonication, etc. Top electropherogram represents the sizing ladder.


Plasmid DNA

Separating the 3 forms of Plasmid DNA can be challenging. Each form -- nicked, supercoiled or linear -- can run differently depending on its' size and dye intercalation. The Fragment Analyzer™ Automated CE System is the only multichannel capillary electrophoresis instrument that can successfully separate the 3 plasmid DNA forms.


Large DNA Fragments

Would you undertake different research projects if you had an automated CE instrument that could separate large fragments?
PCR fragments are not always small in size and sometimes larger amplicons (>1,500bp) need to be analyzed. Some competitive DNA analysis instruments are based on single-strand technology, and are very limited in the fragment sizes they can analyze. The Fragment Analyzer™ Automated CE System platform has several gels for larger fragments, including a gel for efficient separation of fragments <12,000bp and another gel for <40,000bp fragments.


Mutation Detection / TILLiNG

TILLiNG (Targeting Induced Local Lesions in Genomes) is a reverse genetics technique designed to detect unknown mutations in a gene by forming DNA heteroduplex as a result of mixing wild-type and chemically-induced mutated genes.
In order to increase experimental throughput, the genes are pooled first, then PCR amplified. This is followed by heteroduplex formation, then the samples are enzymatically digested. Next, digested cleaved DNA fragments are analyzed for scoring. The enzyme specifically recognizes the mismatched base pairs and cleaves the DNA at the site of mismatch.
Until now, detecting unknown mutations was both a challenging and a time intensive process. Advanced Analytical has developed a streamlined TILLiNG process that requires NO fluorescently labeled primers and NO sample clean-up. Detecting unknown mutations has never been easier, especially with this process developed for the Fragment Analyzer™ Automated CE System platform (see below). In comparison to the traditional method, the Fragment Analyzer™ Automated CE System method requires less than ½ the hands on time and analyses can be completed twice as fast (total time) compared to competitive methods.
Currently, Fragment Analyzer™ Automated CE System customers are using their instruments to do:
• reverse genetics TILLiNG of rice.
• reverse genetics TILLiNG of corn.
• reverse genetics TILLiNG of wheat.
• reverse genetics TILLiNG of lettuce.
• reverse genetics TILLiNG of tomato.
• reverse genetics TILLiNG of soybean.
Regardless of whether your lab wants to do TILLiNG (mutation detection) with diploids, tetraploids or hexaploids, the Fragment Analyzer™ Automated CE System has excellent sensitivity for analyzing medium and large-sized TILLiNG pools.

DNF-900-K0500 dsDNA Reagent Kit, 35-500 bp (500 samples)
  • Gel, Dye and Buffers package
  • Diluent Buffer, Ladder, Qualitative Markers (35 & 500bp)
  • Instruction Manual

DNF-910-K0500
dsDNA Reagent Kit, 35-1,500 bp (500 samples)
  • Gel, Dye and Buffers package
  • Diluent Buffer, Ladder, Qualitative Markers (35 & 1,500bp)
  • Instruction Manual

DNF-910-15L80M-K0500
dsDNA Reagent Kit, 35-5,000 bp (500 samples)
  • Gel, Dye and Buffers package
  • Diluent Buffer, Ladder, Qualitative Markers (35 & 5,000bp)
  • Instruction Manual

DNF-920-K0500
dsDNA Reagent Kit, 75-15,000 bp (500 samples)
  • Gel, Dye and Buffers package
  • Diluent Buffer, Ladder, Qualitative Markers (75 & 15,000bp)
  • Instruction Manual

DNF-930-K0500 dsDNA Reagent Kit, 75-20,000 bp (500 samples)
  • Gel, Dye and Buffers package
  • Diluent Buffer, Ladder, Qualitative Markers (75 & 20,000bp)
  • Instruction Manual

DNF-479-0500
Standard Sensitivity NGS Fragment Analysis Kit, 500 samples
  • Gel and Buffer package
  • DM Buffer (Diluent and 35 bp/6,000 bp Markers)
  • Instruction Manual

DNF-486-0500
High Sensitivity NGS Fragment Analysis Kit, 500 samples
  • Gel and Buffer package
  • DM Buffer (Diluent and 35 bp/6,000 bp Markers)
  • Instruction Manual

DNF-489-0500
Sensitivity RNA Analysis Kit, 500 Samples
  • Gel and Buffer package
  • DM Buffer (Diluent and Lower Marker)
  • Instruction Manual

DNF-491-0500 
High Sensitivity RNA Analysis Kit, 500 samples
  • Gel and Buffer package
  • DM Buffer (Diluent and Lower Marker)
  • Instruction Manual

DNF-487-0500
Standard Sensitivity Genomic DNA Analysis Kit, 500 samples
  • Gel and Buffer package
  • DM Buffer (Diluent and Lower Marker)
  • Instruction Manual

DNF-488-0500
High Sensitivity Genomic DNA Analysis Kit, 500 samples
  • Gel and Buffer package
  • DM Buffer (Diluent and Lower Marker)
  • Instruction Manual

DNF-910-K0500T
Mutation Discovery 910 Gel Kit, 500 samples
  • Gel, Dye and Buffers package
  • Diluent Buffer, Ladder, Qualitative Markers (35 & 5,000bp)
  • Instruction Manual

DNF-920-K0500T
Mutation Discovery 920 Gel Kit, 500 samples
  • Gel, Dye and Buffers package
  • Diluent Buffer, Ladder, Qualitative Markers (35 & 5,000bp)
  • Instruction Manual

DNF-480-3000
dsDNA Cleavage Kit, 3000 samples
  • T-Digest Buffer, dsDNA Cleavage Enzyme and Dilution Buffer

DNF-600-U010
Intercalating Dye, 10uL

DNF-475-0100 5X Capillary Conditioning Solution, 100 mL

DNF-490-0060 10-3 M Fluorescein, 60 uL with diluent

DNF-495-0075 Dilution Buffer E, 1X, 75 mL

GP-440-0100 Capillary Storage Solution, 100 mL

P60-20 Fragment Analyzer 96-Well Buffer/Waste Tray, case of 50

A2300-9650-3355 96-Capillary Array Cartridge 50 um (ID) 33 cm (EFF), 55 cm (TOT), 10mL (RES)

A2300-9650-5580 96-Capillary Array Cartridge 50 um (ID) 55 cm (EFF), 80 cm (TOT), 10mL (RES)

A2300-1250-5580 12-Capillary Array Cartridge 50 um (ID) 55 cm (EFF), 80 cm (TOT), 2mL (RES)

A2300-1250-3355 12-Capillary Array Cartridge 50 um (ID) 33 cm (EFF), 55 cm (TOT), 2mL (RES)
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