Moxi GO 532
3 Parameter Next Gen Flow
Increase the pace of scientific discovery with ORFLO’s next generation Moxi GO 532™ Next Gen Flow Cytometers. The system is equipped with one fluorescent channels (587/30nm ) for cell analysis. Every assay also delivers simultaneous cell count & volume determination, using single-use flow cell. This eliminates the hassle of traditional flow associated with cleaning, maintenance, clearing of clogs, cross contamination and occasionally replacement of bottles and tubes.
Furthermore the Moxi GO 532™ use very little sample volume, 60ul's allowing you to conserver precious, expensive sample, such as stem cells. Cell concentrations as low as 10,000 cells per ml are possible, which most experiments would enable a little as 5ul's of sample diluted in 55ul's of PBS.
The Moxi GO 532™ can be utilized as an assay development instrument and many powerful cell based assays can easily be optimized and run on the system. These include:
- Gold-Standard Counts & Viability
- Cell surface immuno-labeling
- Apoptosis with PE - Annexin V
- Reactive Oxidation Species Experiments
- Rhod AM studies
- In-Cell Protein Quant
- Mito Potential Experiements
The Moxi GO 532™ comes standard with an ultra-intuitive, plug-and-play interface with free OS updates as long as you own the instrument. No prior flow cytometry experience is required you simply just plug and play.
How It Works
The operating principle behind the Moxi GO II Flow Cytometers is a unique combination of Coulter-style cell size determination with simultaneous fluorescence detection. As cells flow single file through the microfabricated single-use flow cell the volume of each particle is measured at the exact same time as their primary fluorescence is measured using a 532nm solid state diode laser with a 580/37 (e.g. PE, PI) emission filter. Thousands of cells are measured in the 10 second read time and the data are plotted in a gradient density scatter plot as Cell size (volume) vs. Fluorescence (PMT voltage). Gating is easily performed on the unit using a interactive touch display, and the resulting live/dead ratios are automatically calculated (depending on the app selected). The analyzed data can also be displayed as a two color size histogram. Total volumetric cell counts are automatically determined for each test by precisely measuring the volume of fluid being analyzed.
Data can be displayed on the unit in both a color density scatter plot and a two color size histogram. Simply drag gates using the intuitive touch display for instant live/dead ratio calculations and each of the gated volumetric cell counts (i.e., total population, live population, and dead population (Viability App). The mean cell volume for the gated populations is also automatically displayed on the unit. Results from each test are stored in the standard FCS 3:1 format and can be viewed using any flow cytometry analysis package. The actual Moxi Flow screenshots from each assay (dot plots and histograms) are also stored in bitmap format for use online. Hundreds of files can be stored on each Moxi GO and are easily transferred to a Mac or PC using USB on-the-go. No aditional software is required.