QuantiSpeed SYBR (No/Low/Hi)-ROX
In one-step RT-qPCR, cDNA synthesis and qPCR are performed in a single reaction vessel in a common reaction buffer with the option of included (No/Low/Hi)ROX
SYBR Green One-Step Kit uses the latest developments in reverse transcriptase technology and buffer chemistry for efficient cDNA synthesis and PCR in a single tube.
Our modified MMLV reverse transcriptase (RTase) is both thermostable and extremely active. The enzyme is blended with RNase inhibitor preventing degradation of RNA by contaminating RNase. The RTase is not inhibited by ribosomal and transfer RNAs, total RNA is an ideal substrate.
SYBR Green mixes use an intercalating dye which does not inhibit PCR, unlike other popular dyes.
SYBR Green One-Step mix uses proprietary small molecular inhibitor technology that prevents formation of primer-dimers to improve reaction sensitivity and specificity.
High-throughput screening has resulted in a buffer system that allows efficient amplification from GC-rich and AT-rich templates, under fast and standard cycling conditions.