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Electrophoresis
Scarlet protein stain
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AdvanStain Scarlet protein stain

AdvanStain Scarlet Protein Stain

AdvanStain Scarlet is a fluorescent stain for gels and blots that allows sensitive and quantitative visualization of proteins. Gels and blots stained with AdvanStain Scarlet can be imaged on any fluorescence imaging system, including laser- and CCD-based systems, with a detection limit of less than 1 ng of protein per band or spot. Fully compatible with downstream Western blotting or mass spectrometry, AdvanStain Scarlet is non-toxic and biodegradable, for safe and simple disposal.

Features
  • Sensitive – detect less than a nanogram of protein per spot
  • Convenient – simple 3-hour protocol
  • Flexible – stain gels or membranes
  • Reversible – destain for downstream Western blotting or mass spectrometry
  • No speckling – clean background for better data
  • Safe – biodegradable and contains no heavy metals for increased safety and simple disposal
  • Compatible – image with any laser or CCD imaging system. Compatible excitation wavelenghts include green (543, 532 nm), blue (488 nm), violet (405 nm) and UV (302, 365 nm). Maximum emission wavelenght is 610 nm
AdvanStain Scarlet is a sensitive, quantitative fluorescent stain for detection of protein on gels or blots. With a quick and easy protocol, gels can be stained in less than 3 hours, and imaged on a wide variety of imaging systems.

AdvanStain_MultipleSystems.jpg

AdvanStain_ExcitationWavelength.jpg

Figure 1
Figure 1. AdvanStain Scarlet can be imaged under multiple imaging conditions, with sensitivity of 1 ng or less per band. The optimal excitation and emission wavelengths of AdvanStain Scarlet are compatible with multiple imaging conditions, and gels stained with AdvanStain Scarlet can be imaged using UV (305 or 365 nm), blue, or green-light excitation, using a transilluminator, CCD- or laser-based imaging system.

AdvanStain_DynamicRange.jpg

Figure 2
Figure 2. AdvanStain Scarlet signal is linear for more than 2 orders of magnitude. Serial dilutions of transferrin protein were analyzed by SDS-PAGE and the gel stained with AdvanStain Scarlet.
Includes:
  • AdvanStain Scarlet Dye Concentrate
  • Powder A, for preparation of Fix Solution
  • Powder B, for preparation of Stain Buffer
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