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Purification
RapidClean
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모델 제품명 size 가격 수량 주문
EA26100 1 Kb Plus DNA Ladder 100 lane, 50ug 로그인 확인 문의
EA31100 dATP, 100mM solution 1 x 1mL 로그인 확인 문의

RapidClean

The fast and easy way to remove protein from RNA and DNA samples

In less than 5 minutes, completely remove protein from aqueous solutions of single- and double-stranded nucleic acids.

Features
  • Complete removal of proteins from aqueous solutions of nucleic acids
  • Protocol less than 5 minutes, start to finish
  • Non-toxic alternative to phenol-chloroform extraction
  • Nucleic acid recovery rates >95%
  • Recovery of nucleic acids from 5 nucleotides to greater than 40 kb
  • Suitable for DNA, RNA, cDNA, microRNA, oligonucleotides

RapidClean is a novel affinity resin designed to remove all protein from aqueous solutions of nucleic acids, providing an alternative to hazardous and lengthy phenol-chloroform procedures. The RapidClean resin binds and removes protein in a 5-minute protocol that combines convenience, speed, and nucleic acid recovery rates in excess of 95%.
RC-Fig1.jpg

Figure 1. RapidClean completely removes T4 DNA ligase. No detectable ligase activity remains in a reaction mix after exraction with RapidClean (lane 2). EcoRI digested λ-DNA (lane C, Control) was incubated for 16 hours at room temperature with T4 DNA ligase (lane 1) or with the same mixture extracted with RapidClean (lane 2).

RC-Fig2.jpg

Figure 2. Extraction with RapidClean completely removes Exonuclease III. ϕX174/HaeIII DNA (Lane M) was digested by a 40 min incubation at 37 °C with 10 units of Exonuclease III (lane 1), but no digestion occured when the enzyme mix was extracted twice with RapidClean before addition of the ϕX174/HaeIII DNA target (lane 2).
Rapid clean resin arrives as a ready-to-use 10x slurry

Rapid clean kit Includes:
  • RapidClean resin
  • Spin filters


Products related to nucleotide electrophoresis:

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